Immunomodulation by Trypanosoma cruzi: Toward Understanding the Association of Dendritic Cells with Infecting TcI and TcII Populations

Figure 2

T. cruzi strains do not alter DC viability. Murine bone marrow-derived DCs were incubated for 18 h with no stimulation (medium), LPS (100 ng/mL), or different T. cruzi strains (MOI 3 : 1), and viable cells were identified by a lack of annexin V or PI staining. (a) Dot plots of representative samples showing the % of viable cells (Annexin V-FITC- and/or PI-DCs, left quadrant). Negative control: unstained DCs. Positive control: previous to annexin V and PI staining, the DCs were maintained for 30 min at 57°C. (b) The % of viable DCs. The bars represent the mean, and the vertical lines represent the standard error; Student’s -test or Mann-Whitney test.

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