Research Article

fMLP-Induced IL-8 Release Is Dependent on NADPH Oxidase in Human Neutrophils

Figure 10

ERK1/2, PI3K, p38 MAPK, and NADPH inhibition reduce p65 NF-κB translocation induced by fMLP. Neutrophils were incubated with vehicle, UO126 (1 μM), LY294002 (10 μM), SB203580 (10 μM), HMAP (500 μM), DPI (10 μM), or SN50 (100 μg/mL) for 30 min and then stimulated with fMLP for 30 min. The cells were fixed and analysed by immunocytochemistry with an antibody targeted against p65 NF-κB. The nucleus was stained with propidium iodide (PI). The arrow shows the presence of p65 NF-κB in nucleus. The negative control was prepared without anti-p65 NF-κB. Bar: 10 m. Figure is representative of three independent experiments. Each bar represents mean ± SE of arbitrary units (AU) of fluorescence intensity of p65 NF-κB, = at least 5; ; compared to fMLP.