fMLP-Induced IL-8 Release Is Dependent on NADPH Oxidase in Human Neutrophils

Figure 3

NADPH oxidase inhibition reduces IL-8 production. Neutrophils were treated with HMAP, DPI, or vehicle for 30 min and stimulated with fMLP for 4 h. IL-8 was measured in the supernatants by ELISA (a). Neutrophils were incubated with fMLP for 1 h, and then 10 μM actinomycin D and 500 μM HMAP, 10 μM DPI, or vehicle were added and incubated for 1 or 3 h. Total RNA was isolated and cDNA synthesis and qPCR of IL-8 and β-actin were done (b). Mean ± SE; ; compared to fMLP; .

(a)

(b)