Journal of Immunology Research / 2015 / Article / Fig 7

Research Article

fMLP-Induced IL-8 Release Is Dependent on NADPH Oxidase in Human Neutrophils

Figure 7

Effects of UO126, LY294002, Akt inhibitor, and SB203580 on IL-8 production and MAPK and Akt phosphorylation induced by fMLP. Neutrophils were incubated with vehicle, UO126 (1 μM), LY294002 (10 μM), SB203580 (10 μM), andrographolide (AP) (50 μM), or Akt inhibitor (10 μM) for 30 min and stimulated with fMLP for 4 h. IL-8 production was analysed in the supernatants by ELISA (a). Neutrophils were incubated with vehicle, UO126 (1 μM), LY294002 (10 μM), or SB203580 (10 μM) (b) for 30 min, stimulated with fMLP for 2 min, and analysed by immunoblot for ERK1/2, p38 MAPK, or Akt (Ser 473) phosphorylation. The blots were stripped and stained antibody specific to the unphosphorylated protein. Data presented are representative of three independent experiments. Mean ± SE; ; compared to fMLP; .

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