fMLP-Induced IL-8 Release Is Dependent on NADPH Oxidase in Human Neutrophils

Figure 8

NAPDH oxidase, NHE, MAPK, and PI3K/Akt inhibitors increase the intracellular IL-8 level in fMLP treated cells. Neutrophils were incubated with vehicle, DPI (10 μM), HMAP (500 μM), amiloride (500 μM), SB203580 (10 μM), UO126 (1 μM), LY294002 (10 μM), or Akt inhibitor (10 μM) for 30 min and stimulated with fMLP (100 nM) for 4 h. IL-8 intracellular content was measured by FACS. Data presented are representative of three independent experiments.