fMLP-Induced IL-8 Release Is Dependent on NADPH Oxidase in Human Neutrophils

Figure 9

Effect of HMAP, DPI, and amiloride on MAPK and Akt phosphorylation induced by fMLP. Neutrophils were incubated with vehicle, HMAP, DPI (a), or amiloride (b) for 30 min, stimulated with fMLP for 2 min, and analysed by immunoblot for ERK1/2, p38 MAPK, or Akt (Ser 473) phosphorylation. The blots were stripped and stained antibody specific to the unphosphorylated protein. Data presented are representative of three independent experiments.

(a)

(b)