Journal of Immunology Research / 2015 / Article / Fig 6

Research Article

Modulation of Th1/Th2 Immune Responses by Killed Propionibacterium acnes and Its Soluble Polysaccharide Fraction in a Type I Hypersensitivity Murine Model: Induction of Different Activation Status of Antigen-Presenting Cells

Figure 6

Cytokines production in cocultures of bone marrow-derived dendritic cells and OVA-primed T lymphocytes. Bone marrow cells from naïve F1 BALB/c x A/J mice were cultured during 10 days with GM-CSF, for dendritic cell differentiation. At 10th day, enriched OVA-primed T lymphocytes, obtained from the spleen of HEW-implanted F1 BALB/c x A/J mice, were added to the cultures. The following in vitro stimuli were performed: P. acnes, PS, or OVA at 10th day; P. acnes or PS, concomitantly to OVA, at 10th day (P. acnes + OVA and PS + OVA, resp.); P. acnes or PS at 10th day and OVA at 11th day (P. acnes → OVA and PS → OVA, resp.). Supernatants were collected at 12th day and submitted to ELISA for IL-5 (a) and IL-17 (b) detection. *; ***.

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