Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity

Figure 1

Human myeloma cell lines (HMCLs) inhibit CD99 expression on normal human osteoblasts (hOBs). Human undifferentiated osteoblasts or hOBs differentiated for 2, 7, 14, or 21 days (Days of diff.), were cultured in the presence or absence of H929 (a) or U266 (b) HMCLs or their conditioned medium, and then were analyzed for western blot analysis to detect the protein levels of CD99. The histograms represent the mean optical density (OD) of CD99 ratio normalized to the OD of -Actin. Data are presented as mean ± SE. The figure shows one representative of three independent experiments.

(a)

(b)