Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity

Figure 6

CD99 agonist monoclonal antibody induces AKT and ERK phosphorylation in normal human osteoblasts (hOBs). hOBs, treated with anti-CD99 agonist monoclonal antibody or with mouse IgG for 0, 2, 5, 10, and 20 minutes, were lysed and analyzed by western blot analysis to detect the protein levels of phosphorylated AKT (p-AKT, upper panel) and ERK (p-ERK, lower panel). The histograms represent the mean optical density (OD) of p-AKT or p-ERK ratio normalized to the OD of total-AKT or total-ERK, respectively. Data are presented as mean ± SE. The figure shows one representative of three independent experiments.