Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity

Figure 7

PI3K and ERK1 K inhibitors rescue Collagen I (COLLI), RUNX2, and JUND upregulation induced by CD99 agonist monoclonal antibody in normal human osteoblasts (hOBs). hOBs pretreated for 30 or 60 minutes with PI3K (Wortmannin) or ERK1 K (PD 98059) inhibitors, respectively, were stimulated for 4 (a) or 6 hours (h) (b) with anti-CD99 agonist monoclonal antibody and then lysed and analyzed by western blotting to detect the protein levels of COLLI, RUNX2, and JUND. The histograms represent the mean optical density (OD) of COLLI, RUNX2, or JUND ratio normalized to the OD of -Actin. Data are presented as mean ± SE. The figure shows one representative of three independent experiments.

(a)

(b)