Induction of Apoptosis Coupled to Endoplasmic Reticulum Stress through Regulation of CHOP and JNK in Bone Marrow Mesenchymal Stem Cells from Patients with Systemic Lupus Erythematosus

Figure 1

Apoptosis of BMSCs from SLE patients. (a) BM-MSCs at passage 4 were isolated and harvested from SLE patients and normal controls, and analyzed by Annexin V-FITC/PI staining. Annexin V-positive cells were 4.1 ± 3.7% in normal controls, 32.3 ± 12.0% in SLE patients (). (b) Western blot analysis of BAX, active caspase-3 and Bcl-2 was performed in BM-MSCs from normal controls and SLE patients. (c) BM-MSCs from normal controls and SLE patients were fixed and stained with antiactive caspase-3. FITC-labeled secondary antibody was used (green fluorescence) Nuclei were stained with DAPI (blue fluorescence). Images were captured by a confocal laser microscope. (Bar represents mean ± SD, compared with the normal group).

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