In Vitro Selection of Cancer Cell-Specific Molecular Recognition Elements from Amino Acid Libraries

Figure 2

Differential cell SELEX overview. A target cell type is incubated with a naïve amino acid library displayed on the surface of a host. Those that do not bind are removed by various screening methods, commonly panning, MACS, or FACS. Those that do bind are amplified and incubated with negative target cells. Those that do not bind are amplified and resubjected to the target cell line. This iterative screening process continues for multiple rounds under increasingly stringent conditions until one or a few strongly binding MREs are obtained. Figure adapted from [75].