IL-12 Inhibits Lipopolysaccharide Stimulated Osteoclastogenesis in Mice
Osteoclastogenesis in the calvarial suture. (a) Histological sections of calvariae excised from mice after daily supracalvarial administrations of PBS, LPS alone (100 g/day), LPS with IL-12 (1.5 g/day), or IL-12 alone for 5 days. The sections were stained for TRAP activity. (b) Numbers of TRAP-positive cells in the calvarial suture. (c) TRAP mRNA levels in the mouse calvariae detected by real-time RT-PCR. Total RNA was isolated from the mouse calvariae after daily supracalvarial injections of PBS, LPS, LPS with IL-12, or IL-12 for 5 days. The TRAP mRNA levels were normalized by the corresponding GAPDH mRNA levels. Results are expressed as means ± SD ( = 6; < 0.05, < 0.01). Differences were detected using Scheffe’s tests.
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