IL-12 Inhibits Lipopolysaccharide Stimulated Osteoclastogenesis in Mice

Figure 5

TNF- mRNA levels in the mouse calvariae detected by real-time RT-PCR. Total RNA was isolated from mice calvariae after daily supracalvarial injections of PBS, LPS alone (100 g/day), LPS with IL-12 (1.5 g/day), or IL-12 alone for 5 days. The mRNA levels of TNF- were normalized by the corresponding GAPDH mRNA levels. Results are expressed as means ± SD ( = 6; < 0.05, < 0.01). Differences were detected using Scheffe’s tests.