Research Article

A Novel PET Imaging Using 64Cu-Labeled Monoclonal Antibody against Mesothelin Commonly Expressed on Cancer Cells

Figure 2

Immunohistochemical analysis of MSLN expression. (a) Expression of MSLN protein in cultured cancer cells detected by immunocytochemistry. BxPC-3, CFPAC-1, and PANC-1 cells were plated in 8-well chamber slides, cultured for 3 days, and incubated with 11-25 mAb followed by treatment with FITC-labeled anti-mouse IgG, Alexa Fluor 594-labeled wheat germ agglutinin (WGA), and DAPI. (b) Expression of MSLN in xenografts. Frozen sections of tumors derived from BxPC-3, CFPAC-1, and PANC-1 were treated with Alexa Fluor 488-labeled 11-25 mAb, Alexa Fluor 594-WGA, and DAPI. Bar: 20 μm.
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