Adsorption of Toll-Like Receptor 4 Agonist to Alum-Based Tetanus Toxoid Vaccine Dampens Pro-T Helper 2 Activities and Enhances Antibody Responses

Figure 2

Effects of adsorption of LPS or MPLA onto TT/alum sensitization on TT-mediated airway allergic responses. (a) Protocol: BALB/c mice sensitized with s.c. TT/alum in the presence or not of LPS (10 μg) or MPLA (10 μg) on days 0 and 7, and challenged with TT i.n. on days 14 and 21. Samples obtained on day 22: (b) total number of cells and (c) eosinophils in BAL; ((d) and (e)) levels of IL-5 and IL-13 in BAL. Data shown as mean ± SE, one-way ANOVA: ^#; different from TT/alum/PBS group (), and experiment was repeated twice.

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