Mesenchymal Stem Cells from Patients with Rheumatoid Arthritis Display Impaired Function in Inhibiting Th17 Cells

Table 2

Gene-specific primers used for real-time PCR.

Gene	Sense primer	Antisense primer
Runx2	5′-AGAGGTACCAGATGGGACTGT-3′	5′-GGTAGCTACTTGGGGAGGATT-3′
PPARγ	5′-TCGACCACGTCAATCCAGAGT-3′	5′-TCGCCTTTGCTTTGGTCAG-3′
TGF-β1	5′-AGCGACTCGCCAGAGTGGTTA-3′	5′-GCAGTGTGTTATCCCTGCTGTCA-3′
IDO	5′-GAATGGCACACGCTATGGAA-3′	5′-CAGACTCTATGAGATCAGGCAGATG-3′
PGE2	5′-TGACCAGAGCAGGCAGATGAA-3′	5′-CCACAGCATCGATGTCACCATAG-3′
IL-6	5′-TGAAAGCAGCAAAGAGGCA-3′	5′-TGGGTCAGGGGTGGTTAT-3′
CCL2	5′-GCTCATAGCAGCCACCTTCATTC-3′	5′-GGACACTTGCTGCTGGTGATTC-3′
GAPDH	5′-GCACCGTCAAGGCTGAGAAC-3′	5′-TGGTGAAGACGCCAGTGGA-3′
FAK	5′-GCCTTAACAATGCGTCAGTTTGACC-3′	5′-TCAGTGTGGTCTCGTCTGCCCAAG-3′
Integrin  β1	5′-GGGAAACTTGGTGGCATTG-3′	5′-GCTCCTTGTAAACAGGCTGAAA-3′
HGF	5′-GAAGGTGAAGGTCGGAGTC-3′	5′-GAAGATGGTGATGGGATTTC-3′
VEGF	5′-CCCTGATGAGATCGAGTACA-3′	5′-AGGAAGCTCATCTCTCCTAT-3′
CXCR4	5′-CCTCCTGCTGACTATTCCCGA-3′	5′-GGAACACAACCACCCACAAGT-3′

Runx2, runt-related transcription factor 2; PPARγ, peroxisome proliferator-activated receptor gamma; TGF-β1, transforming growth factor-β1; IDO, indoleamine 2,3-dioxygenase; PGE2, prostaglandin E2; IL-6, interleukin-6; CCL2, chemokine (C-C motif) ligand 2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; FAK, focal adhesion kinase; HGF, hepatocyte growth factor; VEGF, vascular endothelial growth factor; CXCR4, C-X-C chemokine receptor type 4.