Secretion of S100A8, S100A9, and S100A12 by Neutrophils Involves Reactive Oxygen Species and Potassium Efflux

Figure 5

Inhibition of calgranulin secretion by DPI. Neutrophils were preincubated with the NADPH oxidase inhibitor DPI (10 μM) and then stimulated with 1.5 mg/mL MSU crystals or 10 nM PMA for 60 minutes. (a and b) S100A8/A9 and (c and d) S100A12 in the supernatants were then quantified by ELISA. Results represent the means ± SEM of 5 donors. .

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