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Journal of Immunology Research
Volume 2015, Article ID 296149, 16 pages
http://dx.doi.org/10.1155/2015/296149
Research Article

Secretion of S100A8, S100A9, and S100A12 by Neutrophils Involves Reactive Oxygen Species and Potassium Efflux

Axe de Recherche sur les Maladies Infectieuses et L’immunologie, Centre de Recherche du CHU de Québec-Université Laval, and Département de Microbiologie-Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, QC, Canada G1V 0A6

Received 11 August 2015; Revised 6 November 2015; Accepted 1 December 2015

Academic Editor: Nicolas Demaurex

Copyright © 2015 Mélanie R. Tardif et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

ELISAs specific for S100A8, S100A9, and S100A12 homodimers, as well as S100A8/A9 heterodimer were generated using monoclonal and polyclonal antibodies produced in our laboratory. Using these antibodies, we demonstrated that most of chemotactic factors did not induce thesecretion of S100 proteins. However, MSU crystals induced the secretion of S100A8/A9 and S100A12, and this secretion was inhibited by DPI and high concentrations of K+.

  1. Supplementary Material