Development of Nonaggregating Poly-A Tailed Immunostimulatory A/D Type CpG Oligodeoxynucleotides Applicable for Clinical Use

Table 1

Modified D35 ODNs developed in this study.

D35(CG)G-dAs40	GsGTGCATCGATGCAGGGGsGsGs-As40	Figure 1(a)	1
D35(GC)G-dAs40	GsGTGCATGCATGCAGGGGsGsGs-As40		2
D35(TG)G-dAs40	GsGTGCATTGATGCAGGGGsGsGs-As40		3
D35(CT)G-dAs40	GsGTGCATCTATGCAGGGGsGsGs-As40		4
D35(CG)A-dAs40	GsGTGCATCGATGCAAAAAsAsAs-As40		5
D35(GC)A-dAs40	GsGTGCATGCATGCAAAAAsAsAs-As40		6
D35(TG)A-dAs40	GsGTGCATTGATGCAAAAAsAsAs-As40		7
D35(CT)A-dAs40	GsGTGCATCTATGCAAAAAsAsAs-As40		8
D35(CG)T-dAs40	GsGTGCATCGATGCATTTTsTsTs-As40		9
D35(GC)T-dAs40	GsGTGCATGCATGCATTTTsTsTs-As40		10
D35(TG)T-dAs40	GsGTGCATTGATGCATTTTsTsTs-As40		11
D35(CT)T-dAs40	GsGTGCATCTATGCATTTTsTsTs-As40		12
D35(CG)C-dAs40	GsGTGCATCGATGCACCCCsCsCs-As40		13
D35(GC)C-dAs40	GsGTGCATGCATGCACCCCsCsCs-As40		14
D35(TG)C-dAs40	GsGTGCATTGATGCACCCCsCsCs-As40		15
D35(CT)C-dAs40	GsGTGCATCTATGCACCCCsCsCs-As40		16
D35T-dAs40	GsGTGCATCGATGCATTTTsTsTs-As40	Figure 1(b)	1
D35T-dA40	GsGTGCATCGATGCATTTTsTsTs-A40		2
D35T-dTs40	GsGTGCATCGATGCATTTTsTsTs-Ts40		3
D35T-dT40	GsGTGCATCGATGCATTTTsTsTs-T40		4
D35T-dCs40	GsGTGCATCGATGCATTTTsTsTs-Cs40		5
D35T-dC40	GsGTGCATCGATGCATTTTsTsTs-C40		6
D35-dAs40	GsGTGCATCGATGCAGGGGsGsGs-As40	Figures 3(a), 3(b), and 3(c); Figures 4(a) and 4(b)
D35T-dAs40	GsGTGCATCGATGCATTTTsTsTs-As40
D35core	TGCATCGATGCA
D35core-dAs40	TGCATCGATGCA-As40
D35coreT-dAs40	TGCATCGATGCATTTTsTsTs-As40
D35core-dAs10	TGCATCGATGCA-As10	Figures 4(c) and 4(d)
D35core-dAs20	TGCATCGATGCA-As20
D35core-dAs40	TGCATCGATGCA-As40

“s” indicates phosphorothioate backbone.
D35(CG)G-dAs40 and D35-dAs40 have the same sequence.
D35(CG)T-dAs40 and D35T-As40 have the same sequence.