Differential Immune Response against Recombinant Leishmania donovani Peroxidoxin 1 and Peroxidoxin 2 Proteins in BALB/c Mice
(a) Sequence comparison of Leishmania donovani Pxn1 and Pxn2. Alignment of amino acid sequence depicts the high homology between LdPxn1 and LdPxn2. Highlighted areas show positions of mismatch. LdPxn2 possesses extra 9 amino acids at the carboxy terminus (underlined) that are missing from LdPxn1. (b) SDS-PAGE and western blot of rLdPxn1 and rLdPxn2 proteins. One microgram per lane of rLdPxn1 (lane 1) and rLdPxn2 (lane 2) was separated on a 12% SDS-PAGE and stained with Coomassie blue, top. The separated samples were transferred to Hybond-P membrane and were probed with pooled sera from mice immunized with the respective recombinant protein, bottom. Molecular weight in kDa is shown on the left.