Schematic representation of the immunization protocol. Six- to 8-week-old female BALB/c mice were immunized s.c. in the hind foot pad with recombinant LdPxn1 or LdPxn2 protein (25 ug/mouse) with or without CpG ODN (50 ug/mouse) or GLA-SE (20 ug/mouse) adjuvants. Mice were boosted twice in 3 weeks interval. Sera were collected at each time of injection. Four weeks after the last boost mice were euthanized and sera, lymph nodes, and spleens were collected. Samples were used for antibody and cytokine analysis using standard protocols.