Gender-Related Effects of Sex Steroids on Histamine Release and FcεRI Expression in Rat Peritoneal Mast Cells

Figure 2

Effect of gender and sex steroid concentration on histamine release induced by substance P in mast cells. Rat PMCs were pretreated with E₂, P₄, T₄, and DHT at 10 pM to 10 nM for 90 min at 37°C, followed by 30 min incubation with 10 μM of substance P. Values are means ± SEM of three independent experiments in triplicate. , , and with and without hormone. Comparisons between genders were performed by one-way analysis of variance (ANOVA), followed by Bonferroni’s multiple comparison test (GraphPad Prism). Hormone concentration and gender had significant effect and the interaction is statistically significant ().

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