Dendritic Cell Activity Driven by Recombinant Mycobacterium bovis BCG Producing Human IL-18, in Healthy BCG Vaccinated Adults

Figure 7

Secretion of IFN-γ (a) and IL-10 (b) by human naive (grey bars) and memory (black bars) T cells following 24 h and 96 h coculture with PPD- (10 μg/mL), BCG- (1 : 1), rBCGIL-18- (1 : 1), or LPS- (1 μg/mL) pulsed autologous MoDCs (ratio MoDCs/T cells, 1 : 10). The cytokine levels in the cocultures were measured by ELISA. Data shown are the mean ± SEM of 20 independent donors. Statistical analyses were performed using the Kruskal-Wallis test and Mann-Whitney test. ; .

(a)

(b)