Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip from Herpesvirus saimiri

Figure 5

Tris-Tricine electrophoretic identification of hTip-CSKH or control peptides. (a) 10 μg of the indicated peptides (1 = hTip-CSKH; 2 = hTip; 3 = hTip-CSKH-Scr; 4 = CSKH) was mixed with 10 μL of a protein extract of red blood cells membranes. The gel was stained with Coomassie Brilliant Blue. The size of each molecular weight marker (MWM) is indicated in the right and the control (c) membranes without peptides in the left. The arrow indicates the band corresponding to hTip-CSKH molecular weight. (b) The membrane extract from hTip-CSKH-treated human PBMC was resolved by ultracentrifugation in a sucrose density gradient. Eight fractions were recovered as indicated in each line. Half of each fraction was used for Tris-Tricine electrophoresis for hTip-CSKH identification (the arrow marks the band corresponding to hTip-CSKH molecular weight). The remaining amount of the fraction was used for WB blot in order to know its Flotillin 2 content (bottom) and to differentiate between light (lanes 1–3) and heavy (lanes 5–8) fractions. The size of each molecular weight marker (MWM) is indicated in the left.

(a)

(b)