The Ability of Secretory Leukocyte Protease Inhibitor to Inhibit Apoptosis in Monocytes Is Independent of Its Antiprotease Activity

Figure 1

Effect of SLPI on TNF-α-induced caspase-3 and caspase-7 activity and cell death. U937 monocytic cells were pretreated for 1 hr with SLPI (10 μg/mL) and stimulated with TNF-α (10 ng/mL) for 4 hr. (a) Activity of the effector caspases, caspase-3 and caspase-7, was measured using the fluorogenic substrate DEVD-AMC. Results are representative of . (b) The amount of apoptosis occurring in cells was measured by ELISA. Results are representative of experiments. indicates .

(a)

(b)