Journal of Immunology Research / 2015 / Article / Fig 1

Research Article

Carbohydrate Microarrays Identify Blood Group Precursor Cryptic Epitopes as Potential Immunological Targets of Breast Cancer

Figure 1

Carbohydrate microarray analysis of antiepiglycanin mAb HAE3. Seventy-six glycoproteins, glycoconjugates, and polysaccharides were spotted in triplicates in 1 to 2 dilutions to yield the customized microarrays for antibody screening. (a) Microarray detections were shown as the mean fluorescent intensities (MFIs) of each microspot with antigen-binding signal in red and background reading in blue. Each error bar is constructed using one standard deviation from the mean of triplicate detections. The labeled antigens include HCA (ID# 1 and ID# 2), a number of blood group precursors (29#–32#), and a microarray spotting marker (80#). (b) Images of a microarray stained with HAE3 (5 μg/mL). (c) Schematic of a blood group substance structure with the conserved O-glycan core highlighted.

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.