Journal of Immunology Research / 2015 / Article / Tab 1

Research Article

Carbohydrate Microarrays Identify Blood Group Precursor Cryptic Epitopes as Potential Immunological Targets of Breast Cancer

Table 1

Dataset from a carbohydrate microarray analysis of mAb HAE3 (Figure 1).

Antigen spotted Fluorescent intensities (INT) Microarray scores (-INT)
(ID#) antigen nameReplicatesMeansStDevAg/BgMeansStDev-test ( value)

(1)HCA(A549) 1 : 5323062142.0911.1673040.1325636.66755E − 05
(2) HCA (A549)385372657.7313.0589960.0445971.0582E − 07
(3) Cyst9 (A)3106550.9610.057080.00626270.695270476
(4) JS (A)3105920.9610.0484850.00272460.630836897
(5) HGM-BGS (A + H)31060120.9610.0497850.01661160.641720796
(6) Hog76 (A)3109560.9910.0962640.00726030.982754025
(7) Hog (A)399670.909.96046330.00962330.195275968
(8) MSS (A)311391341.0310.1471310.16510350.701231922
(9) Hog39 B2 (A)31046100.9510.0310840.01363870.512794031
(10) Cyst14 (A2)311441161.0410.1550530.14357730.6354829
(11) WG (A2)31021130.929.99522390.01767860.320320938
(12) Cyst113101120.919.98109040.0021810.261637986
(13) Cow213108390.9810.0807830.01222560.886644093
(14) Beach (B)31152831.0410.1670590.10232480.51871479
(15) Cow28 (B)31065270.9610.0563220.03698580.695618692
(16) Cow433101270.929.98249750.00948430.267256771
(17) Cow2631093560.9910.0923950.07328570.985749244
(18) Hog53105740.9610.0462090.00479070.614466244
(19) Hog 10%3107680.9710.0714370.01045060.809324608
(20) Hog6 (H)314625951.3210.4418030.54359610.384564394
(21) Hog6731067130.9710.0588220.01766940.709880521
(22) Hog (H)31132101.0210.1446230.01231780.598292687
(23) Hog3031071400.9710.0640740.05396680.760857847
(24) Cow21 (I-Ma)3103170.9310.0093390.00994030.386688505
(25) Cow2531051240.9510.0368360.0331840.555735986
(26) Cow26 (I)31008130.919.9776830.0178860.250673214
(27) N-1 10% 2X (Le)31139101.0310.1535120.01319090.537783247
(28) N-1 IO4- (Le)31044180.9410.02730.02504170.491711028
(29) Beach P1 (Ii)32119221.9211.0491180.014760.000114629
(30) McDon P1376756046.9512.9030320.1135794.91829E − 08
(31) TijII (Ii)370502206.3812.7829430.0447741.83548E − 07
(32) OG (Ii)31116643310.1013.4460470.0565952.50433E − 08
(33) LNT-BSA (Type I)31092120.9910.0927030.01524730.9870917
(34) Pn XIV (Type II)31108171.0010.1136240.022650.838256847
(35) ASOR (Tri/m-II)31128261.0210.1388730.03309740.644502626
(36) AGOR (Tri/m-Gn)3104250.9410.025590.00681880.477740236
(37) iAFGP3102450.9310.0004580.00709240.342706487
(38) Chondroitin-SO4-A31038130.9410.0190530.01793510.440853108
(39) Chondroitin-SO4-B31128101.0210.1399390.01286020.631919148
(40) Chondroitin-SO4-C31047140.9510.0319630.01890580.519441046
(41) Hyaluronic Acid3109690.9910.0984410.01135820.964347497

(79) Bg611051881.0010.094230.219076
(80) Dex70K-FITC363225106757.2215.9480710.0243011

Antigen’s initial spotting concentration was 0.5 μg/μL. The positive results are emphasized with bold. Microbial antigens tested (4278) were negative with HAE3 (Ag/Bg < 1.20; > 0.20, data not shown).
-test: microarray scores, that is, the -transformed microarray values from triplicate spots for each antigen, were applied in a -test to examine the differences of significance between each probe and Bg (microarray reading background), which is the mean fluorescent intensity of six spots of Av-Cy3/Cy5 (ID# 79) in the FITC channel.

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