Carbohydrate Microarrays Identify Blood Group Precursor Cryptic Epitopes as Potential Immunological Targets of Breast Cancer
Table 1
Dataset from a carbohydrate microarray analysis of mAb HAE3 (Figure 1).
Antigen spotted
Fluorescent intensities (INT)
Microarray scores (-INT)
(ID#) antigen name
Replicates
Means
StDev
Ag/Bg
Means
StDev
-test ( value)
(1)HCA(A549) 1 : 5
3
2306
214
2.09
11.167304
0.132563
6.66755E − 05
(2) HCA (A549)
3
8537
265
7.73
13.058996
0.044597
1.0582E − 07
(3) Cyst9 (A)
3
1065
5
0.96
10.05708
0.0062627
0.695270476
(4) JS (A)
3
1059
2
0.96
10.048485
0.0027246
0.630836897
(5) HGM-BGS (A + H)
3
1060
12
0.96
10.049785
0.0166116
0.641720796
(6) Hog76 (A)
3
1095
6
0.99
10.096264
0.0072603
0.982754025
(7) Hog (A)
3
996
7
0.90
9.9604633
0.0096233
0.195275968
(8) MSS (A)
3
1139
134
1.03
10.147131
0.1651035
0.701231922
(9) Hog39 B2 (A)
3
1046
10
0.95
10.031084
0.0136387
0.512794031
(10) Cyst14 (A2)
3
1144
116
1.04
10.155053
0.1435773
0.6354829
(11) WG (A2)
3
1021
13
0.92
9.9952239
0.0176786
0.320320938
(12) Cyst11
3
1011
2
0.91
9.9810904
0.002181
0.261637986
(13) Cow21
3
1083
9
0.98
10.080783
0.0122256
0.886644093
(14) Beach (B)
3
1152
83
1.04
10.167059
0.1023248
0.51871479
(15) Cow28 (B)
3
1065
27
0.96
10.056322
0.0369858
0.695618692
(16) Cow43
3
1012
7
0.92
9.9824975
0.0094843
0.267256771
(17) Cow26
3
1093
56
0.99
10.092395
0.0732857
0.985749244
(18) Hog5
3
1057
4
0.96
10.046209
0.0047907
0.614466244
(19) Hog 10%
3
1076
8
0.97
10.071437
0.0104506
0.809324608
(20) Hog6 (H)
3
1462
595
1.32
10.441803
0.5435961
0.384564394
(21) Hog67
3
1067
13
0.97
10.058822
0.0176694
0.709880521
(22) Hog (H)
3
1132
10
1.02
10.144623
0.0123178
0.598292687
(23) Hog30
3
1071
40
0.97
10.064074
0.0539668
0.760857847
(24) Cow21 (I-Ma)
3
1031
7
0.93
10.009339
0.0099403
0.386688505
(25) Cow25
3
1051
24
0.95
10.036836
0.033184
0.555735986
(26) Cow26 (I)
3
1008
13
0.91
9.977683
0.017886
0.250673214
(27) N-1 10% 2X (Le)
3
1139
10
1.03
10.153512
0.0131909
0.537783247
(28) N-1 IO4- (Le)
3
1044
18
0.94
10.0273
0.0250417
0.491711028
(29) Beach P1 (Ii)
3
2119
22
1.92
11.049118
0.01476
0.000114629
(30) McDon P1
3
7675
604
6.95
12.903032
0.113579
4.91829E − 08
(31) TijII (Ii)
3
7050
220
6.38
12.782943
0.044774
1.83548E − 07
(32) OG (Ii)
3
11166
433
10.10
13.446047
0.056595
2.50433E − 08
(33) LNT-BSA (Type I)
3
1092
12
0.99
10.092703
0.0152473
0.9870917
(34) Pn XIV (Type II)
3
1108
17
1.00
10.113624
0.02265
0.838256847
(35) ASOR (Tri/m-II)
3
1128
26
1.02
10.138873
0.0330974
0.644502626
(36) AGOR (Tri/m-Gn)
3
1042
5
0.94
10.02559
0.0068188
0.477740236
(37) iAFGP
3
1024
5
0.93
10.000458
0.0070924
0.342706487
(38) Chondroitin-SO4-A
3
1038
13
0.94
10.019053
0.0179351
0.440853108
(39) Chondroitin-SO4-B
3
1128
10
1.02
10.139939
0.0128602
0.631919148
(40) Chondroitin-SO4-C
3
1047
14
0.95
10.031963
0.0189058
0.519441046
(41) Hyaluronic Acid
3
1096
9
0.99
10.098441
0.0113582
0.964347497
(79) Bg
6
1105
188
1.00
10.09423
0.219076
(80) Dex70K-FITC
3
63225
1067
57.22
15.948071
0.0243011
Antigen’s initial spotting concentration was 0.5 μg/μL. The positive results are emphasized with bold. Microbial antigens tested (42–78) were negative with HAE3 (Ag/Bg < 1.20; > 0.20, data not shown). -test: microarray scores, that is, the -transformed microarray values from triplicate spots for each antigen, were applied in a -test to examine the differences of significance between each probe and Bg (microarray reading background), which is the mean fluorescent intensity of six spots of Av-Cy3/Cy5 (ID# 79) in the FITC channel.
