Neutrophil Migration in the Activation of the Innate Immune Response to Different Flavobacterium psychrophilum Vaccines in Zebrafish (Danio rerio)

Figure 2

Effect on innate immune response triggered by freshly prepared vaccines against F. psychrophilum. ((a)–(j)) Representative images of zebrafish tails from larvae at 58 hpf. ((k) and (l)) Graph quantifying the number of neutrophils outside of the CHT (ROI), showing the effect on the innate immune response in larvae bath-vaccinated with (k) heat inactivated bacteria or (l) formaldehyde. Statistical analysis was performed using two-way ANOVA. All embryos ((a)–(j)) were oriented anterior to the left, with the dorsal on top. Data represent the mean ± standard error from three independent experiments with 15 larvae each. value < 0.05, , and .

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