SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis

Figure 6

Colocalization of SP140L, SP140, SP110, and SP100A with PML and p300. The nuclear body protein PML does not colocalize with SP140L (a–c), SP140 (d–f), or SP110 (g–i) in HeLa cells. PML colocalizes with its known interaction partner SP100A (j–l). Similarly, the transcriptional coactivator p300 does not colocalize with SP140L (m–o), SP140 (p–r), or SP110 (s–u). Cytofluorograms together with Pearson’s correlation coefficients for the overlap of red and green pixel intensities correspond to the images on their left (v). SP140L was detected with monoclonal anti-Myc antibody and anti-FLAG antibody was used to detect SP140, SP110, and SP100A. Polyclonal anti-HA antibody was used to stain HA-PML and HA-p300. Scale bar: 5 μm.