SP140L, an Evolutionarily Recent Member of the SP100 Family, Is an Autoantigen in Primary Biliary Cirrhosis

Table 1

Clinical and autoantibody data of studied 22 PBC patients.

Patient	Age	Stage	AMA	ANA	SP100 (LIPS)	SP140 (LIPS)	SP140L (LIPS)
P1	50	III	1 : 100	—	+	+	—
P2	51	II	—	1 : 100	+	+	+
P3	54	II	1 : 320	—	—	—	—
P4	63	IV	1 : 100	—	+	—	—
P5	54	IV	1 : 40	—	—	—	—
P6	44	III	1 : 320	—	+	—	—
P7	88	III	1 : 320	—	—	+	—
P8	43	II	1 : 100	—	—	—	—
P9	63	IV	1 : 160	—	—	—	—
P10	65	III	1 : 100	—	—	—	—
P11	66	II	1 : 100	1 : 10	—	—	—
P12	42	III	1 : 100	—	—	—	—
P13	61	II	1 : 100	—	+	+	+
P14	—	IV	1 : 8000	—	+	—	—
P15	79	III	1 : 4000	—	+	—	+
P16	64	III	1 : 8000	—	+	—	—
P17	59	II	1 : 8000	—	—	—	—
P18	70	II	1 : 1000	—	+	—	—
P19	49	IV	1 : 500	—	+	—	—
P20	60	I	1 : 8000	—	—	—	—
P21	—	II	—	—	—	—	—
P22	72	III	1 : 200	—	—	—	—

PBC staging was performed by liver biopsy sample histology. Indirect immunofluorescence on rodent tissue slides was used for AMA (antimitochondrial antibodies) and ANA (antinuclear antibodies) detection at indicated serum dilutions. The LIPS assay is described in Methods.