Local and Systemic IKK and NF-B Signaling Associated with Sjögren’s Syndrome Immunopathogenesis
Protein expression of pIKKε, total IKKε, pIKKα/β, and pNF-κB p65 were significantly increased in PBMC from patients with pSS, compared to healthy controls. (a) Phosphorylated and total protein expression of IKKε, IKKα/β, IKKγ, IκBα, and NF-κB p65 in PBMC from two healthy controls (H) and two representative pSS patients was shown by western blot. (b) The relative expression of pIKKε, total IKKε, pIKKα/β, and pNF-κB p65 were higher in pSS (), compared to healthy controls (). β-actin was used as endogenous control, and relative expression of each protein is shown as protein/β-actin ratio. ;. All data are representative of three independent experiments.