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Journal of Immunology Research
Volume 2015, Article ID 538171, 12 pages
http://dx.doi.org/10.1155/2015/538171
Research Article

Administration of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934, Two Strains Isolated from Human Milk, to Very Low and Extremely Low Birth Weight Preterm Infants: A Pilot Study

1Departamento Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain
2Servicio de Neonatología, Hospital Universitario La Paz, 28046 Madrid, Spain
3Probisearch, Tres Cantos, 28760 Madrid, Spain

Received 18 July 2014; Revised 8 September 2014; Accepted 16 September 2014

Academic Editor: Miguel Gueimonde

Copyright © 2015 Laura Moles et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. F. Magne, M. Abély, F. Boyer, P. Morville, P. Pochart, and A. Suau, “Low species diversity and high interindividual variability in faeces of preterm infants as revealed by sequences of 16S rRNA genes and PCR-temporal temperature gradient gel electrophoresis profiles,” FEMS Microbiology Ecology, vol. 57, no. 1, pp. 128–138, 2006. View at Publisher · View at Google Scholar · View at Scopus
  2. Y. Wang, J. D. Hoenig, K. J. Malin et al., “16S rRNA gene-based analysis of fecal microbiota from preterm infants with and without necrotizing enterocolitis,” ISME Journal, vol. 3, no. 8, pp. 944–954, 2009. View at Publisher · View at Google Scholar · View at Scopus
  3. C. Rougé, O. Goldenberg, L. Ferraris et al., “Investigation of the intestinal microbiota in preterm infants using different methods,” Anaerobe, vol. 16, no. 4, pp. 362–370, 2010. View at Publisher · View at Google Scholar · View at Scopus
  4. M. S. LaTuga, J. C. Ellis, C. M. Cotton et al., “Beyond bacteria: a study of the enteric microbial consortium in extremely low birth weight infants,” PLoS ONE, vol. 6, no. 12, Article ID e27858, 2011. View at Publisher · View at Google Scholar · View at Scopus
  5. S. Arboleya, A. Binetti, N. Salazar et al., “Establishment and development of intestinal microbiota in preterm neonates,” FEMS Microbiology Ecology, vol. 79, no. 3, pp. 763–772, 2012. View at Publisher · View at Google Scholar · View at Scopus
  6. J. C. Hallab, S. T. Leach, L. Zhang et al., “Molecular characterization of bacterial colonization in the preterm and term infant's intestine,” Indian Journal of Pediatrics, vol. 80, no. 1, pp. 1–5, 2013. View at Publisher · View at Google Scholar · View at Scopus
  7. L. Moles, M. Gómez, H. Heilig et al., “Bacterial diversity in meconium of preterm neonates and evolution of their fecal microbiota during the first month of life,” PLoS ONE, vol. 8, no. 6, Article ID e66986, 2013. View at Publisher · View at Google Scholar · View at Scopus
  8. R. Martín, A. J. Nauta, K. Ben Amor, L. M. Knippels, J. Knol, and J. Garssen, “Early life: gut microbiota and immune development in infancy,” Beneficial microbes, vol. 1, no. 4, pp. 367–382, 2010. View at Publisher · View at Google Scholar · View at Scopus
  9. J. L. Kaplan, H. N. Shi, and W. A. Walker, “The role of microbes in developmental immunologic programming,” Pediatric Research, vol. 69, no. 6, pp. 465–472, 2011. View at Publisher · View at Google Scholar · View at Scopus
  10. P. van Baarlen, J. M. Wells, and M. Kleerebezem, “Regulation of intestinal homeostasis and immunity with probiotic lactobacilli,” Trends in Immunology, vol. 34, no. 5, pp. 208–215, 2013. View at Publisher · View at Google Scholar · View at Scopus
  11. A. A. Sharma, R. Jen, A. Butler, and P. M. Lavoie, “The developing human preterm neonatal immune system: a case for more research in this area,” Clinical Immunology, vol. 145, no. 1, pp. 61–68, 2012. View at Publisher · View at Google Scholar · View at Scopus
  12. E. C. Claud and W. A. Walker, “Hypothesis: inappropriate colonization of the premature intestine can cause neonatal necrotizing enterocolitis,” The FASEB Journal, vol. 15, no. 8, pp. 1398–1403, 2001. View at Publisher · View at Google Scholar · View at Scopus
  13. M.-F. de la Cochetière, H. Piloquet, C. des Robert, D. Darmaun, J.-P. Galmiche, and J.-C. Rozé, “Early intestinal bacterial colonization and necrotizing enterocolitis in premature infants: the putative role of Clostridium,” Pediatric Research, vol. 56, no. 3, pp. 366–370, 2004. View at Publisher · View at Google Scholar · View at Scopus
  14. M.-J. Butel, A. Suau, F. Campeotto et al., “Conditions of bifidobacterial colonization in preterm infants: a prospective analysis,” Journal of Pediatric Gastroenterology and Nutrition, vol. 44, no. 5, pp. 577–582, 2007. View at Publisher · View at Google Scholar · View at Scopus
  15. J. C. Madan, R. C. Salari, D. Saxena et al., “Gut microbial colonisation in premature neonates predicts neonatal sepsis,” Archives of Disease in Childhood: Fetal and Neonatal Edition, vol. 97, no. 6, pp. F456–F462, 2012. View at Publisher · View at Google Scholar · View at Scopus
  16. A. Janvier, J. Malo, and K. J. Barrington, “Cohort study of probiotics in a North American neonatal intensive care unit,” Journal of Pediatrics, vol. 164, no. 5, pp. 980–985, 2014. View at Publisher · View at Google Scholar · View at Scopus
  17. N. D. Embleton and T. Skeath, “Probiotics for preterm infants on the NICU,” Paediatrics and Child Health, vol. 24, no. 1, pp. 38–40, 2014. View at Publisher · View at Google Scholar · View at Scopus
  18. Q. Wang, J. Dong, and Y. Zhu, “Probiotic supplement reduces risk of necrotizing enterocolitis and mortality in preterm very low-birth-weight infants: an updated meta-analysis of 20 randomized, controlled trials,” Journal of Pediatric Surgery, vol. 47, no. 1, pp. 241–248, 2012. View at Publisher · View at Google Scholar · View at Scopus
  19. G. Deshpande, S. Rao, S. Patole, and M. Bulsara, “Updated meta-analysis of probiotics for preventing necrotizing enterocolitis in preterm neonates,” Pediatrics, vol. 125, no. 5, pp. 921–930, 2010. View at Publisher · View at Google Scholar · View at Scopus
  20. W. O. Tarnow-Mordi, D. Wilkinson, A. Trivedi, and J. Brok, “Probiotics reduce all-cause mortality and necrotizing enterocolitis: it is time to change practice,” Pediatrics, vol. 125, no. 5, pp. 1068–1070, 2010. View at Publisher · View at Google Scholar · View at Scopus
  21. H.-C. Lin, C.-H. Hsu, H.-L. Chen et al., “Oral probiotics prevent necrotizing enterocolitis in very low birth weight preterm infants: a multicenter, randomized, controlled trial,” Pediatrics, vol. 122, no. 4, pp. 693–700, 2008. View at Publisher · View at Google Scholar · View at Scopus
  22. M. G. Romeo, D. M. Romeo, L. Trovato et al., “Role of probiotics in the prevention of the enteric colonization by Candida in preterm newborns: Incidence of late-onset sepsis and neurological outcome,” Journal of Perinatology, vol. 31, no. 1, pp. 63–69, 2011. View at Publisher · View at Google Scholar · View at Scopus
  23. R. Mohan, C. Koebnick, J. Schildt et al., “Effects of Bifidobacterium lactis Bb12 supplementation on intestinal microbiota of preterm infants: a double-blind, placebo-controlled, randomized study,” Journal of Clinical Microbiology, vol. 44, no. 11, pp. 4025–4031, 2006. View at Publisher · View at Google Scholar · View at Scopus
  24. Y. Ohashi and K. Ushida, “Health-beneficial effects of probiotics: its mode of action,” Animal Science Journal, vol. 80, no. 4, pp. 361–371, 2009. View at Publisher · View at Google Scholar · View at Scopus
  25. M. Millar, M. Wilks, P. Fleming, and K. Costeloe, “Should the use of probiotics in the preterm be routine?” Archives of Disease in Childhood: Fetal and Neonatal Edition, vol. 97, no. 1, pp. F70–F74, 2012. View at Publisher · View at Google Scholar · View at Scopus
  26. E. A. Cristofalo, R. J. Schanler, C. L. Blanco et al., “Randomized trial of exclusive human milk versus preterm formula diets in extremely premature infants,” The Journal of Pediatrics, vol. 163, no. 6, pp. 1592.e1–1595.e1, 2013. View at Publisher · View at Google Scholar · View at Scopus
  27. A. F. Ahrabi and R. J. Schanler, “Human milk is the only milk for premies in the NICU!,” Early Human Development, vol. 89, no. 2, pp. S51–S53, 2013. View at Publisher · View at Google Scholar · View at Scopus
  28. D. I. Tudehope, “Human milk and the nutritional needs of preterm infants,” Journal of Pediatrics, vol. 162, no. 3, pp. S17–S25, 2013. View at Publisher · View at Google Scholar · View at Scopus
  29. I. Levy, J. Comarsca, M. Davidovits, G. Klinger, L. Sirota, and N. Linder, “Urinary tract infection in preterm infants: the protective role of breastfeeding,” Pediatric Nephrology, vol. 24, no. 3, pp. 527–531, 2009. View at Publisher · View at Google Scholar · View at Scopus
  30. R. J. Schanler, C. Lau, N. M. Hurst, and E. O. Smith, “Randomized trial of donor human milk versus preterm formula as substitutes for mothers' own milk in the feeding of extremely premature infants,” Pediatrics, vol. 116, no. 2, pp. 400–406, 2005. View at Publisher · View at Google Scholar · View at Scopus
  31. R. Martín, M. Olivares, M. L. Marín, L. Fernández, J. Xaus, and J. M. Rodríguez, “Probiotic potential of 3 lactobacilli strains isolated from breast milk,” Journal of Human Lactation, vol. 21, no. 1, pp. 8–17, 2005. View at Publisher · View at Google Scholar · View at Scopus
  32. R. Martín, E. Jiménez, H. Heilig et al., “Isolation of bifidobacteria from breast milk and assessment of the bifidobacterial population by PCR-denaturing gradient gel electrophoresis and quantitative real-time PCR,” Applied and Environmental Microbiology, vol. 75, no. 4, pp. 965–969, 2009. View at Publisher · View at Google Scholar · View at Scopus
  33. L. Fernández, S. Langa, V. Martín et al., “The human milk microbiota: origin and potential roles in health and disease,” Pharmacological Research, vol. 69, no. 1, pp. 1–10, 2013. View at Publisher · View at Google Scholar · View at Scopus
  34. M. Gueimonde, K. Laitinen, S. Salminen, and E. Isolauri, “Breast milk: a source of bifidobacteria for infant gut development and maturation?” Neonatology, vol. 92, no. 1, pp. 64–66, 2007. View at Publisher · View at Google Scholar · View at Scopus
  35. G. Solís, C. G. de los Reyes-Gavilan, N. Fernández, A. Margolles, and M. Gueimonde, “Establishment and development of lactic acid bacteria and bifidobacteria microbiota in breast-milk and the infant gut,” Anaerobe, vol. 16, no. 3, pp. 307–310, 2010. View at Publisher · View at Google Scholar · View at Scopus
  36. S. Arboleya, P. Ruas-Madiedo, A. Margolles et al., “Characterization and in vitro properties of potentially probiotic Bifidobacterium strains isolated from breast-milk,” International Journal of Food Microbiology, vol. 149, no. 1, pp. 28–36, 2011. View at Publisher · View at Google Scholar · View at Scopus
  37. V. Martín, A. Maldonado-Barragán, L. Moles et al., “Sharing of bacterial strains between breast milk and infant feces,” Journal of Human Lactation, vol. 28, no. 1, pp. 36–44, 2012. View at Publisher · View at Google Scholar · View at Scopus
  38. M. Mshvildadze, J. Neu, J. Shuster, D. Theriaque, N. Li, and V. Mai, “Intestinal microbial ecology in premature infants assessed with non-culture-based techniques,” Journal of Pediatrics, vol. 156, no. 1, pp. 20–25, 2010. View at Publisher · View at Google Scholar · View at Scopus
  39. C. Greenwood, A. L. Morrow, A. J. Lagomarcino et al., “Early empiric antibiotic use in preterm infants is associated with lower bacterial diversity and higher relative abundance of Enterobacter,” The Journal of Pediatrics, vol. 165, no. 1, pp. 23–29, 2014. View at Publisher · View at Google Scholar · View at Scopus
  40. A. Blanco-Quirós, E. Arranz, G. Solis, A. Villar, A. Ramos, and D. Coto, “Cord blood interleukin-10 levels are increased in preterm newborns,” European Journal of Pediatrics, vol. 159, no. 6, pp. 420–423, 2000. View at Publisher · View at Google Scholar · View at Scopus
  41. N. Matoba, N. Yu, K. Mestan et al., “Differential patterns of 27 cord blood immune biomarkers across gestational age,” Pediatrics, vol. 123, no. 5, pp. 1320–1328, 2009. View at Publisher · View at Google Scholar · View at Scopus
  42. S. Lusyati, C. V. Hulzebos, J. Zandvoort, H. Sukandar, and P. J. J. Sauer, “Cytokines patterns in newborn infants with late onset sepsis,” Journal of Neonatal-Perinatal Medicine, vol. 6, no. 2, pp. 153–163, 2013. View at Publisher · View at Google Scholar · View at Scopus
  43. N. Takahashi, R. Uehara, M. Kobayashi et al., “Cytokine profiles of seventeen cytokines, growth factors and chemokines in cord blood and its relation to perinatal clinical findings,” Cytokine, vol. 49, no. 3, pp. 331–337, 2010. View at Publisher · View at Google Scholar · View at Scopus
  44. G. Hodge, S. Hodge, R. Haslam et al., “Rapid simultaneous measurement of multiple cytokines using 100 μl sample volumes—Association with neonatal sepsis,” Clinical and Experimental Immunology, vol. 137, no. 2, pp. 402–407, 2004. View at Publisher · View at Google Scholar · View at Scopus
  45. S. Josefsson, S. K. Bunn, and M. Domellöf, “Fecal calprotectin in very low birth weight infants,” Journal of Pediatric Gastroenterology and Nutrition, vol. 44, no. 4, pp. 407–413, 2007. View at Publisher · View at Google Scholar · View at Scopus
  46. G. Aydemir, F. Cekmez, I. A. Tanju et al., “Increased fecal calprotectin in preterm infants with necrotizing enterocolitis,” Clinical Laboratory, vol. 58, no. 7-8, pp. 841–844, 2012. View at Publisher · View at Google Scholar · View at Scopus
  47. K. Suzuki, S.-A. Ha, M. Tsuji, and S. Fagarasan, “Intestinal IgA synthesis: a primitive form of adaptive immunity that regulates microbial communities in the gut,” Seminars in Immunology, vol. 19, no. 2, pp. 127–135, 2007. View at Publisher · View at Google Scholar · View at Scopus
  48. T. Rogosch, S. Kerzel, K. Hoß et al., “IgA response in preterm neonates shows little evidence of antigen-driven selection,” The Journal of Immunology, vol. 189, no. 11, pp. 5449–5456, 2012. View at Publisher · View at Google Scholar · View at Scopus
  49. V. Mai, R. M. Torrazza, M. Ukhanova et al., “Distortions in development of intestinal microbiota associated with late onset sepsis in preterm infants,” PLoS ONE, vol. 8, no. 1, Article ID e52876, 2013. View at Publisher · View at Google Scholar · View at Scopus
  50. V. Mai, C. M. Young, M. Ukhanova et al., “Fecal microbiota in premature infants prior to necrotizing enterocolitis,” PLoS ONE, vol. 6, no. 6, Article ID e20647, 2011. View at Publisher · View at Google Scholar · View at Scopus
  51. R. H. Siggers, J. Siggers, T. Thymann, M. Boye, and P. T. Sangild, “Nutritional modulation of the gut microbiota and immune system in preterm neonates susceptible to necrotizing enterocolitis,” Journal of Nutritional Biochemistry, vol. 22, no. 6, pp. 511–521, 2011. View at Publisher · View at Google Scholar · View at Scopus