Table 3: Conservativeness of epitopic PV peptide regions among 43 PV strains and variants.

PV strain UniProt/Swiss-Prot entry aa Pos 464 aa Pos 708 aa Pos 748 aa Pos 763 aa Pos 783 aa Pos 835 aa Pos 847 PV1 Mahoney P03300 FMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1 Sabin P03301 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV3 P3/Leon/37 P03302 SMMATG RFDME sWDDYTWQT FYTYG AYSHFYD KvRiY VWCPR PV3 23127 P06209 SMMATG RFDME sWDDYTWQT FYTYG AYSHFYD KvRvY VWCPR PV1 isolated Q9Q281 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KvRVY VWCPR PV1 isolated Q9Q280 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1 isolated Q71AZ9 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1 isolated Q5TLH5 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-HAI01008C2 D1YSI9 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-HAI01009 D1YSJ1 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-HAI01008 D1YSJ2 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-HAI01002 D1YSJ3 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-HAI01001 D1YSJ4 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR01012 D1YSJ5 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR01002C D1YSJ6 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR01002 D1YSJ7 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR01001C1 D1YSJ9 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR00042C2 D1YSK1 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR00042C1 D1YSK2 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR00042 D1YSK3 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR00041C3 D1YSK4 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR00041C2 D1YSK5 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR00044 D1YSK6 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR00028C D1YSK7 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR00028 D1YSK8 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR00023C D1YSK9 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR00025 D1YSL0 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOOR24 D1YSL1 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR00015 D1YSL2 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-DOR00016 D1YSL3 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-HAI01013all D1YSL4 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-HAI01015 D1YSL5 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV1-S302 D2X673 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR CHN8184/GZ/CHN/2004 D8L541 SMMATG RFDME KWDDYTWQT FYTYG AYSHFYD KIRVY VWCPR PV3 isolated B4YUL3 SMMATG RFDME sWDDYTWQT FYTYG AYSHFYD KvRVY VWCPR PV3 isolated B4YUL4 SMMATG RFDME sWDDYTWQT FYTYG AYSHFYD KvRVY VWCPR PV3 (vacc.StrainSabin3 (Leon 12a1b)) Q84792 SMMATG RFDME sWDDYTWQT FYTYG AYSHFYD KvRiY VWCPR PV3-33239 C5HJY2 SMMATG RFDME sWDDYTWQT FYTYG AYSHFYD KvRiY VWCPR PV3-31974 C5HJY3 SMMATG RFDME sWDDYTWQT FYTYG AYSHFYD KvRiY VWCPR PV3-FIN84-60212 D1GE40 SMMATG RFDME sWDDYTWQT FYTYG AYSHFYD KvRiY VWCPR PV3-SWI10947 D2E679 SMMATG RFDME sWDDYTWQT FYTYG AYSHFYD KvRiY VWCPR PV3-FIN84-2493 D1GE41 SMMATG RFDME sWDDYTWQT FYTYG AYSHFYD KvRiY VWCPR PV3-P3/Jinan/1/09 D2XUS9 SMMATG RFDME sWDDYTWQT FYTYG AYSHFYD KvRiY VWCPR

The 43 PV sequences were aligned using ClustalW multialignment program (http://www.uniprot.org/align/) [24]. The analyzed PV sequences and related references are described at http://www.uniprot.org/ and reported by Swiss-Prot/UniProtKB accession number. Peptide sequences present in PV-derived epitopes (see Table 2) and absent in the human proteome were localized along the 43 aligned PV sequences and analyzed for conservativeness. Peptide sequences are indicated by their position along the PV polyprotein. Mutated aa residues are in lower case.