Vitamin D Deficiency Contributes to the Reduction and Impaired Function of Naïve Regulatory T Cell in Chronic Heart Failure

Figure 5

1,25(OH)2D elevated Foxp3 expression while it inhibits IL-17A in CD4⁺CD45RA⁺T cells. (a) Representative flow cytometric dot plots and diagrams of IFN- and IL-17 or Foxp3 and IL-17 double staining in CD4⁺T cell after 5 days of culture with 1,25(OH)2D or DMSO. (b) CD4⁺CD45RA⁺T and CD4⁺CD45RA⁻T cells were purified from HD. IL-17, IFN-, or Foxp3 expressions were detected by FACS after 7 days of culture. (c) CD4⁺CD45RA⁺T cells were purified from HD and CHF. IL-17, IFN-, or Foxp3 expressions were detected by FACS after 7 days of culture. (d) Foxp3, IL-17, and RORγt mRNA expressions in CD4⁺CD45RA⁺T cells after 7 days of culture, CD45RA⁺CD4⁻T cells as negative control. (e) HD CD4⁺CD45RA⁺T cells culture supernatant was collected on days 0, 1, 3, 5, and 7 and detected IL-10, IL-17, and IFN- by ELISA. (f) CD4⁺CD45RA⁺T cells were purified from HD and CHF. IL-10, IL-17, and IFN- were detected by ELISA on day 7. Data are representative of five independent experiments. Results shown are the mean ± SEM; ^*.

(a)

(b)

(c)

(d)

(e)

(f)