Journal of Immunology Research / 2015 / Article / Fig 6

Research Article

Vitamin D Deficiency Contributes to the Reduction and Impaired Function of Naïve Regulatory T Cell in Chronic Heart Failure

Figure 6

1,25(OH)2D induced CD4+CD45RA+T cells prohibited CD4+CD25 T effector cells via upregulated VDR. VDR expression in CD4+T (a) or in CD4+CD45RA+T and CD4+CD45RAT cells (b) was measured by FACS PBL from PBL of HD and CHF. (c) CD4+T cells were isolated by MACS and then incubated with a series of concentrations of 1,25(OH)2D for 5 days. VDR and Foxp3 expression in induced cells was measured by WB. (d) CD4+CD45RA+T cells were isolated by MACS and incubated with 1,25(OH)2D or DMSO for 5 days. VDR expression was measured by WB and real-time PCR.

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