Stimulation of Innate and Adaptive Immunity by Using Filamentous Bacteriophage fd Targeted to DEC-205

Figure 1

Schematic representation of the engineered filamentous bacteriophage fdOVA/sc-αDEC displaying single chain variable fragment (scFv) anti-DEC-205 molecules as fusion to pIII proteins and the peptide as N-terminal fusion to pVIII proteins. The scFv is composed of heavy (VH) and light (VL) chain variable regions of the mouse monoclonal antibody NLDC145, assembled with a (Gly4Ser)3 linker to yield a single-chain fragment binding the mouse dendritic cells DEC-205 receptor. About 9200 bases of engineered genome include about 6400 bases of wild type DNA, the β-lactamase gene conferring the Ampicillin resistance, one additional copy of pVIII gene plus bases coding for the peptide, and the sequence encoding for the single chain antibody fragment anti-DEC-205 plus the HA tag.