Regulation of Murine Ovarian Epithelial Carcinoma by Vaccination against the Cytoplasmic Domain of Anti-Müllerian Hormone Receptor II

Figure 1

Generation of recombinant mouse AMHR2-CD. (a) Schematic representation of full-length AMHR2 showing the extracellular, transmembrane, and cytoplasmic domains with a C-terminal 6xHis-tagged AMHR2-CD variant. (b) Expression of AMHR2-CD in noninduced, IPTG-induced, and Ni-NTA affinity purified AMHR2-CD shown on an SDS-PAGE gel stained with Coomassie blue. (c) Anti-His Western blot of SDS-PAGE gel showing expression of AMHR2-CD in noninduced, IPTG-induced, and two doses of Ni-NTA affinity purified AMHR2-CD.

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