Research Article

Regulation of Murine Ovarian Epithelial Carcinoma by Vaccination against the Cytoplasmic Domain of Anti-Müllerian Hormone Receptor II

Table 1

Primer pairs used for cloning, qRT-PCR, detection of transgene, and conventional RT-PCR.

ProteinSequence (5′-3′)Amplicon length (bp)

Cloning of AMHR2-CD
AMHR2-CD
 ForwardGGATCCAAGGCCTGCAGAGTGCAAGGTG1209
 ReverseAAGCTTCTACTCATTTACATACACCTG
TgMISIIR-TAg transgene expression
SV40-TAg
 ForwardTGCATGGTGTACAACATTCC773
 ReverseTTGGGACTGTGAATCAATGCC
qRT-PCR
IFN
 ForwardGGATATCTGGAGGAACTGGCAA110
 ReverseTGATGGCCTGATTGTCTTTCAA
TNFα
 ForwardCGAGTGACAAGCCTGTAGCC209
 ReverseGTGGGTGAGGAGCACGTAGT
IL-2
 ForwardGCAGGCCACAGAATTGAAAG207
 ReverseTCCACCACAGTTGCTGACTC
CD4
 ForwardACACACCTGTGCAAGAAGCA69
 ReverseGCTCTTGTTGGTTGGGAATC
CD8
 ForwardTTACATCTGGGCACCCTTG132
 ReverseTTGCCTTCCTGTCTGACTAGC
NKR-P1A
 ForwardGGCTTGGCATGAGTCACC75
 ReverseTTCAGAGCCAACCTGTGTGA
β-actin
 ForwardGGTCATCACTATTGGCAACG133
 ReverseACGGATGTCAACGTCACACT
Conventional RT-PCR
AMHR2
 ForwardGTATCCGCTGCCTCTACAGC193
 ReverseCAGAAGTCAGTGCCACAGGA
β-actin
 ForwardGGTCATCACTATTGGCAACG133
 ReverseACGGATGTCAACGTCACACT