Research Article
Regulation of Murine Ovarian Epithelial Carcinoma by Vaccination against the Cytoplasmic Domain of Anti-Müllerian Hormone Receptor II
Table 1
Primer pairs used for cloning, qRT-PCR, detection of transgene, and conventional RT-PCR.
| Protein | Sequence (5′-3′) | Amplicon length (bp) |
| Cloning of AMHR2-CD | | | AMHR2-CD | | | Forward | GGATCCAAGGCCTGCAGAGTGCAAGGTG | 1209 | Reverse | AAGCTTCTACTCATTTACATACACCTG | TgMISIIR-TAg transgene expression | | | SV40-TAg | | | Forward | TGCATGGTGTACAACATTCC | 773 | Reverse | TTGGGACTGTGAATCAATGCC | qRT-PCR | | | IFN | | | Forward | GGATATCTGGAGGAACTGGCAA | 110 | Reverse | TGATGGCCTGATTGTCTTTCAA | TNFα | | | Forward | CGAGTGACAAGCCTGTAGCC | 209 | Reverse | GTGGGTGAGGAGCACGTAGT | IL-2 | | | Forward | GCAGGCCACAGAATTGAAAG | 207 | Reverse | TCCACCACAGTTGCTGACTC | CD4 | | | Forward | ACACACCTGTGCAAGAAGCA | 69 | Reverse | GCTCTTGTTGGTTGGGAATC | CD8 | | | Forward | TTACATCTGGGCACCCTTG | 132 | Reverse | TTGCCTTCCTGTCTGACTAGC | NKR-P1A | | | Forward | GGCTTGGCATGAGTCACC | 75 | Reverse | TTCAGAGCCAACCTGTGTGA | β-actin | | | Forward | GGTCATCACTATTGGCAACG | 133 | Reverse | ACGGATGTCAACGTCACACT | Conventional RT-PCR | | | AMHR2 | | | Forward | GTATCCGCTGCCTCTACAGC | 193 | Reverse | CAGAAGTCAGTGCCACAGGA | β-actin | | | Forward | GGTCATCACTATTGGCAACG | 133 | Reverse | ACGGATGTCAACGTCACACT |
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