The Pathogenicity of Anti-β2GP1-IgG Autoantibodies Depends on Fc Glycosylation

Table 1

Antibodies employed for each ELISA.

ELISA	Detection-antibody	Dilution
Anti-β2GP1-IgG	HRP-conjugated rabbit F(ab′) 2 anti-human-IgG	1 : 20.000
Anti-β2GP1-IgG subclasses	HRP-conjugated mouse anti-human IgG1, 2, 3 and 4 (Clones HP6001, HP6002, HP6050 & HP6025)	1 : 20.000
Anti-β2GP1-IgM	HRP-conjugated goat anti-human-IgM	1 : 20.000
Anti-β2GP1-bound C3c	HRP-conjugated sheep anti-C3c	1 : 500
Anti-β2GP1-bound C1q	HRP-conjugated sheep anti-C1q	1 : 500

: purchased from Southern Biotech, Birmingham, Alabama, USA.
: purchased from Abcam, Cambridge, United Kingdom.
HRP = horseradish peroxidase.