Research Article

Renal and Hematological Effects of CLCF-1, a B-Cell-Stimulating Cytokine of the IL-6 Family

Figure 2

Total pSTAT3 in (a) peripheral blood cells and (b) renal cortex was determined by Western blot analysis of lysates as described in Methods and Materials. Values are expressed as the ratio of pSTAT to β-actin. Results of a single experiment are shown. Note that the maximum increase in pSTAT3 is nearly 8-fold greater in kidney cortex compared to blood cells. (c) Mouse spleen mononuclear cells were isolated and analyzed as described in Methods and Materials. Results are expressed as percentage of B cells expressing IgG1 (CD19+/IgG1+). Mean of 3 determinations in each of the 2 mice was used to calculate values, expressed as mean ± standard deviation. CD19+/IgG1+ cells were increased after infusion of CLCF-1. .
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