Research Article

Renal and Hematological Effects of CLCF-1, a B-Cell-Stimulating Cytokine of the IL-6 Family

Figure 3

(a) Isolated rat glomeruli were incubated with the indicated concentrations of CLCF-1 for 15 minutes at 37°C. Anti-CLCF-1 antibody abrogated this effect with maximum effect at antibody concentration of 50 µg/mL. at each concentration. Values are mean ± SEM. , versus control. (b) JAK inhibition of CLC effect on . Isolated rat glomeruli were incubated with 10 ng/mL CLCF-1 for 10 minutes at 37°C either with or without pretreatment by 5 nM JAK2 inhibitor BMS911543 for 15 min at 37°C. Additional glomeruli were treated with BMS911543 alone. CLCF-1 increased was blocked by pretreatment with BMS911543. versus BMS911543 alone or CLCF-1 after BMS911543.
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