Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity

Figure 1

Immunoreactivity against isolated antigens. Representative western blot analysis of PyL antigens obtained through immunoaffinity chromatography with immobilized IgGs from malaria surviving ICR mice. (a) shows the specificity of immune sera from ICR mice against purified antigens followed by the incubation with the secondary antibody anti-mouse IgG/HRP linked Fab and (b) was incubated only with the secondary antibody. Lanes were loaded with 10 μg of protein.

(a)

(b)