Experimental Immunization Based on Plasmodium Antigens Isolated by Antibody Affinity

Figure 5

Humoral response of BALB/c mice after immunization with purified PyL antigens and Freund’s adjuvant. The reactivity of sera obtained from mice previously immunized with highly affinity-purified antigens plus Freund’s adjuvant () was assayed on immunoblots of total P. yoelii blood-stage antigen (10 μg/lane) at days 40 (a), 70 (b) and 100 (c). A representative nonvaccinated mouse serum (Control) served as the negative control at corresponding day. Two weeks after last immunization mice were infected with PyL and mouse number survived the infection and mouse number lived until day 17 pi. Antigen-specific antibodies were detected with anti-mouse IgG/HRP linked F(ab).

(a)

(b)

(c)