EPIPOX: Immunoinformatic Characterization of the Shared T-Cell Epitome between Variola Virus and Related Pathogenic Orthopoxviruses

Table 4

Experimentally identified T-cell epitopes within the shared T-cell epitome predicted from pathogenic orthopoxvirus proteins.

(a)

CD8 T-cell epitopes	VARV GI	VARV ORF	Experimental HLA I restriction	Predicted HLA I restriction
ALMRRIAVV	439013	F5R	HLA-A2	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206	HLA-A6802
YLLSLFSTL	439056	A35L	HLA-A2	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206	HLA-A6802
YLAKLTALV	438985	H5R	HLA-A2	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206	HLA-A6802
NLLCHIYSL	438979	K7L	HLA-A2	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206	HLA-Cw0702
IVIEAIHTV	439072	J2R	HLA-A0201	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206	HLA-Cw0304
SLSAYIIRV	439004	I3L	HLA-A0201	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206	HLA-A0207
YLDGQLARL	438965	E6R	HLA-A0201	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206	HLA-A0207
YLPEVISTI	438988	H7L	HLA-A0201	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206	HLA-Cw0102
TYNDHIVNL	439072	J2R	HLA-A2301	HLA-A2301	HLA-A2402	HLA-A2403	HLA-A2405	HLA-A2407	HLA-Cw0702
RPPSFYKPL	439046	A25R	HLA-B7	HLA-B0702	HLA-B3501	HLA-B5101	HLA-B5301	HLA-B5401	HLA-Cw0102
ALDEKLFLI	439045	A24R	HLA-A0201	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206	HLA-A0207
FPYEGGKVF	438968	E9L	HLA-B0702	HLA-B0702	HLA-B1502	HLA-B3501	HLA-B5101	HLA-B5301	HLA-B5401
RLYDYFTRV	438973	K1L	HLA-A0201, HLA-A2	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206
ILDDNLYKV	438985	H5R	HLA-A0201, HLA-A2	HLA-A0201	HLA-A0202	HLA-A0205	HLA-A0207	HLA-Cw0702
LLSYYVVYV	439009	F1R	HLA-A2	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206
FLIDLAFLI	438960	E1L	HLA-A2	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206	HLA-Cw0304
FPRSMLSIF	438994	M4R	HLA-B07:02	HLA-B0702	HLA-B3501	HLA-B4402	HLA-B5301	HLA-B5401
YLFDFVISL	438996	L1R	HLA-A2	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206
YLIKLIEPV	439009	F1R	HLA-A0201, HLA-A2	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206
SPSNHHILL	439025	A4L	HLA-B07:02	HLA-B0702	HLA-B3501	HLA-B5101	HLA-B5301	HLA-B5401
YPSNKNYEI	439033	A12R	HLA-B07:02	HLA-B0702	HLA-B3501	HLA-B5101	HLA-B5301	HLA-B5401
MLMETMFFI	439007	I6R	HLA-A2	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206
ILNPVASSL	438998	L3R	HLA-A2	HLA-A0201	HLA-A0202	HLA-A0205	HLA-A0206	HLA-B1501
FPSVFINPI	438968	E9L	HLA-B0702	HLA-B0702	HLA-B3501	HLA-B5101	HLA-B5301	HLA-B5401
KYQSPVNIF	439043	A21R	HLA-A24, HLA-class I	HLA-A2301	HLA-A2402	HLA-A2403	HLA-A2405	HLA-A2407
YLFGGFSTL	438980	K8R	HLA-A2	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206
YLYETYHLI	438981	H1L	HLA-A2	HLA-A0201	HLA-A0202	HLA-A0205	HLA-A0206
VLYNGVNYL	439009	F1R	HLA-A2	HLA-A0201	HLA-A0202	HLA-A0205	HLA-A0206
LIQEIVHEV	439029	A8L	HLA-A0201	HLA-A0201	HLA-A0202	HLA-A0205	HLA-A0206
VELGSGNSF	439043	A21R	HLA-B3701	HLA-B1501	HLA-B1502	HLA-B4402	HLA-Cw0304
RMIAISAKV	438934	P1L	HLA-A2	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0206
FILGIIITV	439036	A15L	HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0205	HLA-A0206
LLSKNTFYL	438981	H1L	HLA-A2	HLA-A0201	HLA-A0202	HLA-A0205	HLA-A0207
RPRDAIRFL	438961	E2L	HLA-B0702	HLA-B0702	HLA-B3501	HLA-B5301	HLA-B5401
KLFNKVPIV	438996	L1R	HLA-A2	HLA-A0201	HLA-A0202	HLA-A0206	HLA-A6802
SLFMILCTR	438979	K7L	HLA-A0301, HLA-A1101	HLA-A1101	HLA-A3101	HLA-A3301	HLA-A6801
ILNDEQLNL	439029	A8L	HLA-A0201	HLA-A0201	HLA-A0205	HLA-A0207
YLLGDSDSV	439038	A17L	HLA-A2	HLA-A0201	HLA-A0205	HLA-A0206
QLMYALEPR	438985	H5R	HLA-A0301, HLA-A1101	HLA-A3101	HLA-A3301	HLA-A6801
LMDENTYAM	439066	A45R	HLA-A2	HLA-A0205	HLA-A0207	HLA-Cw0702
GLLLGCFWV	438955	C17L	HLA-A2	HLA-A0202	HLA-A0203	HLA-A0206
LLSHFYPAV	438952	C14L	HLA-A2	HLA-A0201	HLA-A0205	HLA-A6802
NLFTFLHEI	439045	A24R	HLA-class I	HLA-A0201	HLA-A0203	HLA-A0206
GLFDFVNFV	439070	A49R	HLA-A2, HLA-A0201	HLA-A0202	HLA-A0203	HLA-A0206
YLGPRVCWL	439038	A17L	HLA-A2	HLA-A0202	HLA-A0205	HLA-A0206
ILKSLGFKV	438980	K8R	HLA-A2	HLA-A0202	HLA-A0203	HLA-A0205
DEVASTHDW	439025	A4L	HLA-B4403	HLA-B4402	HLA-B5801
FLVIAINAM	439029	A8L	HLA-A2, HLA-A0201	HLA-A0206	HLA-Cw0304
LSDLKKTIY	439040	A19R	HLA-A1, HLA-class I	HLA-A0101	HLA-B1501
LLYFKVFGI	439019	N1L	HLA-A2	HLA-A0202	HLA-A0205
SSNIMSESY	439014	F6R	HLA-A0101, HLA-A3002	HLA-A0101	HLA-B1501
DTRGIFSAY	439032	A11L	HLA-A26, HLA-class I	HLA-A0101	HLA-B5801
QIDVEKKIV	439029	A8L	HLA-A0201	HLA-A0207	HLA-A6802
YLFRCVDAV	439030	A9R	HLA-A2	HLA-A0205	HLA-A0206
KIEDLINQL	438973	K1L	HLA-A0201	HLA-A0203	HLA-A0207
FTIDFKLKY	439009	F1R	HLA-A1, HLA-A2601, HLA-A2902	HLA-A0101	HLA-Cw0702
WLKIKRDYL	439074	J4R	HLA-B0801	HLA-B0801
AINVEKIEL	473688	L6R	HLA-A0201	HLA-A0207
RIFVRVYNV	439007	I6R	HLA-A2	HLA-A0202
SIIDLIDEY	438942	C4R	HLA-B1501	HLA-A0203
EERHIFLDY	439009	F1R	HLA-B4403	HLA-B4402
ILSDENYLL	439028	A7L	HLA-A2, HLA-A0201	HLA-A0205
HISALKRRY	439027	A6R	HLA-A0101, HLA-A2902	HLA-A0101
FLNISWFYI	438968	E9L	HLA-A2, HLA-A02:01	HLA-A0205
SEVKFKYVL	439002	I1L	HLA-B44	HLA-B4402
KLLLWFNYL	438980	K8R	HLA-A2	HLA-A0203
YIDISDVKV	438973	K1L	HLA-A0201	HLA-A0207
VWINNSWKF	439013	F5R	HLA-A24, HLA-A2301, HLA-A2402	HLA-Cw0702
VLPFDIKKL	439014	F6R	HLA-A0201	HLA-Cw0102
VETSISDYY	439043	A21R	HLA-B3701	HLA-B1501
SQIIDISLR	439022	A1L	HLA-A0301, HLA-A1101	HLA-B2705
HDVYGVSNF	439045	A24R	HLA-B4403	HLA-B4402

(b)

CD4 T-cell epitope

VARV GI

VARV ORF

Experimental HLA II restriction

Predicted HLA II restriction

FLIDLAFLI

438960

E1L

HLA-class II

DRB10101

DRB10102

DRB10301

DRB10311

DRB10401

DRB11201

DRB11302

DRB11502

DRB30101

DRB50101

IHWQIISSE

439072

J2R

DRB104:05

DRB10405

DRB10406

DRB10410

DRB11304

DRB30101

YIDAYVSRL

439021

N3L

DRB115:01

DRB10901

DRB11103

DRB30202

LMDENTYAM

439066

A45R

HLA-class II

DRB11103

IDAYVSRLL

439021

N3L

DRB115:01

DRB11201

RMIAISAKV

438934

P1L

HLA-class II

DRB10407

T-cell epitopes in this table are a subset of those provided in Additional File S1.