Research Article

Respiratory Syncytial Virus Nonstructural Proteins Upregulate SOCS1 and SOCS3 in the Different Manner from Endogenous IFN Signaling

Figure 2

Nonstructural proteins NS1, NS2, and NS1/2 induced SOCS expression in A549 cells. A549 cells were transfected with NS1, NS2, NS1/2-expressing plasmids, or empty vector at final concentrations of 10 μg/mL, and then total cell lysates were subjected to Western blot analysis using the following antibodies: anti-SOCS1 (1 : 500) and anti-SOCS3 (1 : 500) at the indicated times. (a) pNS1 transfection upregulated the expression levels of SOCS1 and SOCS3 proteins. (b) pNS2 transfection upregulated the expression levels of SOCS1 but SOCS3 proteins. (c) pNS1/2 transfection upregulated the expression levels of SOCS1 and SOCS3 proteins. The data were normalized to the level of β-actin and then to the same protein at time 1 h and are shown as fold increases in density compared with time 1 h from three independent experiments.
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