Statins Increase the Frequency of Circulating CD4⁺FOXP3⁺ Regulatory T Cells in Healthy Individuals

Figure 1

Statins increase the frequency of CD4⁺FOXP3⁺ Treg and mRNA FOXP3. The frequency (a) and absolute number (b) of CD4⁺FOXP3⁺ cells were evaluated by flow cytometry. Methylation percentage in the Treg cell-specific-demethylated-region (TSDR) of FOXP3 gene, in DNA of PBMC from individuals before treatment and 30 days after ongoing treatment, is shown (c). FOXP3 mRNA expression relative to UBC housekeeping gene (d). Median, interquartile range (IQR), and values are shown in the graph; difference between days was tested by Wilcoxon signed-rank test.

(a)

(b)

(c)

(d)