DC-Based Immunotherapy Combined with Low-Dose Methotrexate Effective in the Treatment of Advanced CIA in Mice

Figure 2

Treatment of advanced CIA mice with smDCs alone or in combination with MTX. (a) DBA/1J mice were injected with CFA-mixed type II collagen (CII) on days 0 and 21. Advanced CIA mice (RA score 2~3) were then treated with smDC and/or MTX for 2 weeks. Disease progression was monitored for 132 days. (b) Histological analysis of the hind paws. Hind paws were removed from mice in all groups at day 77 after first CII injection and fixed in paraffin blocks. Histopathology was examined after H&E staining as described in Section 2.

(a)

(b)