Chemokine Receptor Expression on Normal Blood CD56+ NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell Population
Table 1
Chemokine receptor expression on the well-known CD56+low and CD56+high NK-cells observed in blood, as identified based only on the levels of CD56 and CD16 expression.
CD56+low NK-cells
CD56+high NK-cells
values
CXCR1
% (+) cells
93.0 ± 4.5
(85.3–99.6)
4.0 ± 3.6
(0.0–12.1)
<0.001
MFI
100.1 ± 19.4
(79.3–140.9)
7.0 ± 3.1
(2.0–14.5)
<0.001
CV
67.1 ± 23.6
(51.5–145.3)
156.8 ± 76.2
(75.3–329.7)
<0.001
CXCR2
% (+) cells
91.9 ± 3.4
(86.1–97.0)
2.0 ± 1.4
(0.0–4.7)
<0.001
MFI
165.7 ± 68.6
(66.9–264.1)
3.6 ± 1.5
(2.2–6.8)
<0.001
CV
64.1 ± 8.7
(48.5–78.6)
218.1 ± 131.4
(82.7–568.9)
<0.001
CXCR3
% (+) cells
15.6 ± 11.1
(4.8–40.1)
97.0 ± 2.5
(92.5–99.6)
<0.001
MFI
15.5 ± 10.7
(6.5–41.6)
94.5 ± 55.1
(48.7–231.1)
<0.001
CV
209.8 ± 62.9
(145.1–403.4)
62.7 ± 8.1
(49.6–75.3)
<0.001
CXCR4
% (+) cells
21.8 ± 8.7
(8.4–43.5)
11.4 ± 4.6
(5.5–21.4)
<0.001
MFI
11.8 ± 3.9
(5.3–20.9)
7.8 ± 3.1
(3.6–14.0)
n.s.
CV
308.5 ± 89.8
(182.6–492.8)
318.6 ± 98.8
(184.4–581.1)
n.s.
CCR4
% (+) cells
0.8 ± 0.4
(0.2–1.5)
3.3 ± 2.9
(0.3–9.7)
<0.05
MFI
2.2 ± 0.6
(1.3–3.0)
3.1 ± 1.1
(1.5–4.4)
n.s.
CV
393.3 ± 195.1
(138.1–677.6)
118.7 ± 107.2
(59.1–417.3)
0.01
CCR5
% (+) cells
13.3 ± 8.8
(2.8–33.2)
50.0 ± 15.3
(24.8–78.5)
<0.001
MFI
11.1 ± 6.0
(3.5–20.6)
27.8 ± 18.6
(7.7–79.9)
<0.01
CV
353.5 ± 92.8
(194.8–492.5)
133.9 ± 28.1
(96.7–192.4)
<0.001
CCR6
% (+) cells
0.6 ± 0.4
(0.1–1.3)
0.9 ± 1.2
(0.0–3.0)
n.s.
MFI
2.3 ± 0.7
(1.5–3.9)
2.1 ± 0.8
(1.4–4.0)
n.s.
CV
214.7 ± 126.1
(61.4–432.8)
106.7 ± 73.5
(46.2–272.3)
n.s.
Data were obtained using the gating and analysis strategies described in Figure 1, where representative dot plots of these two conventional NK-cell subsets are presented. Results are expressed as mean ± standard deviation (minimum–maximum) of the percentage of cells expressing each of the chemokine receptors analyzed within each CD56+ NK-cell population as well as mean ± standard deviation (minimum–maximum) of the mean fluorescence intensity (MFI) and coefficient of variation (CV) of expression. n.s.: not statistically significant.
