Research Article

Chemokine Receptor Expression on Normal Blood CD56+ NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell Population

Table 3

CD56 and CD16 expression on blood CD56+low CCR5/CXCR3, CD56+int CCR5/CXCR3+, and CD56+high CCR5/CXCR3+ NK-cell subsets.

ABC values
CD56+low CCR5/CXCR3CD56+int CCR5/CXCR3+CD56+high CCR5/CXCR3+A versus BB versus CA versus C

CD56% (+) cells100.0 ± 0.0(100.0-100.0)100.0 ± 0.0(100.0-100.0)100.0 ± 0.0(100.0-100.0)
MFI466 ± 108(303–623)615 ± 149(416–999)2926 ± 578(2161–3993)0.022<0.001<0.001
CV57 ± 7(41–66)72 ± 11(53–93)37 ± 5(27–48)0.001<0.001<0.001

CD16% (+) cells99.9 ± 0.1(99.9–100.0)64.6 ± 23.6(26.8–96.2)28.7 ± 9.9(9.9–43.5)<0.001<0.001<0.001
MFI226 ± 107(55–372)84 ± 55(25–201)47 ± 18(21–47)0.0010.026<0.001
CV45 ± 5(38–55)79 ± 21(45–122)81 ± 21(44–118)<0.001n.s.<0.001

Data were obtained using the gating and analysis strategies described in Figure 2, where representative dot plots of these three NK-cell subsets are presented.
Results are expressed as mean ± standard deviation (minimum–maximum) of the percentage of positive (+) cells within each CD56+ NK-cell population and of the mean fluorescence intensity (MFI) and coefficient of variation (CV) of CD16 and CD56 expression.
n.s.: not statistically significant.